The clinical value of T lymphocyte subsets and NK cells in peripheral blood of patients with high-risk HPV infection and different cervical lesions

Objective: To investigate the clinical value of T lymphocyte subsets and NK cells in peripheral blood of patients with high-risk HPV infection and different cervical lesions. Methods: CD3+, CD4+, CD8+ lymphocyte subsets and CD16+CD56+ cells in specimens of peripheral blood of 212 healthy control and 2167 patients with high-risk HPV infection and different cervical lesions were detected by flow cytometry. Results: Different patients with highrisk HPV infection in cervical lesions, CD4+, CD16+CD56+ cells and the numbers of CD4+/CD8+ ratio were lower than those in the healthy control group, and with the severity of lesions increment to gradually reduce (P<0.01), while CD8+ cell numbers were higher than those in the healthy control group, and with increasing severity lesions gradually increased (P<0.01). The cellular immune function of all patients with cervical squamous cell carcinoma were significantly lower than those in healthy control group, and with the severity of lesions increment to gradually reduce. The cellular immune function of patients of cervical squamous cell carcinoma with lymph node metastasis were significantly lower than that in patients without lymph node metastasis (P<0.01), and in patients with well differentiated, poorly differentiated and moderately differentiated of cervical squamous cell carcinoma group were no difference between them (P>0.05). But CD4+, CD8+ cells and CD4+/CD8+ ratio of patients with low differentiation are significantly lower than patients with well-differentiated. The number of cells of CD4+, CD16+CD56+ reducing and CD4+/CD8+ ratio decreased were negative correlated to clinical stages of cervical squamous cell carcinoma, namely were decreased with the increasing of clinical stages (P<0.01). The number of CD8+ cell was positive correlated with clinical stages of cervical squamous cell carcinoma (P<0.01). Conclusion: The cellular immune function of the patients with high-risk HPV infection and different cervical lesions is lower and especially even worse in patients with cervical squamous cell carcinoma and with advanced clinical stages. Detecting T lymphocyte subsets and NK immune cells can be used to monitor cellular immune function of patients with cervical squamous cell carcinoma and to estimate prognosis and provide reference for clinical treatment.